As per WHO 2010 guidelines, volume below 1 mL is termed as Hypospermia. However, we have observed good count and motility in samples even below 1mL. So, please do not stress about producing an adequate quantity of sample. However, many a time patients mistake pre-ejaculate for ejaculate. Your andrologist will be trained to tell you the difference and may ask you to produce another sample on a later day if the sample that has been produced does not suffice in anyway.
Yes, you may bring the sample from home if 1) you collect the sample in the sterile container provided by the clinic 2) you reach the clinic in 30-40 minutes post collection. Also, your clinic may require you to sign a form of consent declaring that the sample bought from home is your own.
Containers provided by the pathology lab are sterile BUT meant for urine collection so it may be difficult for you to collect semen in them. Containers provided by fertility clinics are specifically designed for semen collection.
Absolutely not, please follow your normal routine. In fact, have a good breakfast or lunch to maintain your energy levels.
Most pathology labs use glass slides to check the semen count. Fertility clinics use a special counting chamber called the Makler or Neubers chamber, specifically meant for checking the sperm count and motility, and it is therefore more accurate.
Commercial condoms have lubricants which are spermicidal. Therefore, it is not advisable to use them. There are special condoms available for the collection of semen samples and can be procured on request.
Please inform the andrologist immediately. Do not collect the sample and put it in the container. The idea is to use sterile sample for your procedure to avoid infection.
Each container is well-labelled and as a practice only one sample is handled at a time.
The samples are discarded as per protocol.
The sample should ideally be collected by masturbation. If you suffer from penile erectile dysfunction or any other related problems, please inform the consultant.
Yes, they do influence the semen parameters. However, it is also something that varies between individuals, and a semen analysis is required to assess the severity in a case.
It is normal to see a slight variation between tests.
Reports are made for all ART procedures and as patients, please ask your clinic for written reports of the same.
There are no restrictions on the bladder.
The time you take to collect the sample is irrelevant; the time is noted at the collection of the sample.
It is uncommon, but you may be asked to collect an additional sample if the andrologist feels that:
Immotile does not necessary mean dead. Sperms can have no motility but they may still be viable to induce pregnancy. There are tests available to determine the difference.
Depending on the investigation done by doctors, it may be deduced that you may only require a little help through IUI to achieve pregnancy. Also, the cost and number of injections to be taken for an IVF cycle are higher.
Abnormal fertilization can be caused by many factors – poor quality oocyte or sperm or in some cases, two sperms may have entered the oocyte, but that phenomenon is rare in ICSI as only a single sperm is injected.
Yes, it is low. Normal fertilization rates are usually 70%. This may be more prevalent in semen samples with low morphology or women with Polycystic Ovary Syndrome (PCOS) where the number of oocytes is high but the quality is poor.
This means that there has been no fertilization. This may be due to extremely poor sperm or egg quality. Please consult your embryologist and clinician for more treatment options.
Abnormal PN may arrest or progress into beautiful embryos, but if transferred these embryos can cause miscarriages and have a higher probability of becoming an abnormal baby.
Evidence has shown that only follicles with sizes of 18-20 mm have mature oocytes. Follicles, if retrieved at a smaller size may have immature oocytes.
Embryologists work according to the embryo development; checks will be done on schedule irrespective of holidays or Sundays.
No, years of research have shown that if the oocytes had to, they would have shown the signs of fertilization between 16-19 hours. So, keeping them for additional hours will not help.
It is not advisable to use the embryo as it may not implant, or may result in a miscarriage or worse, give birth to an abnormal baby.
It takes both the egg and the sperm to make good embryos. Speak to your clinician and the embryologist about the reason for the low-quality of the embryos.
You need to believe both. The grade is dynamic and changes every day. That is why it is important to understand that had you opted for a Day-2-transfer, you would have picked any of the two B grade embryos in their cleavage stage after which it would grow and float in the uterus for two days until it becomes a blastocyst and then implants. All embryos have to progress up to the blastocyst stage before they can implant and culminate in pregnancy. But now, on Day 3, you have discovered that the Day-2-embryo is not necessarily a good one.
Waiting for Day 3 thus gives you:
Paternal Age does not have an impact on count, motility and morphology. However, there may be an increase of DFI (DNA fragmentation index) in such patients.
Oocyte vitrification is done in the following cases:
Vitrification is done by washing the oocytes or embryos in media within a stipulated time. Once the washing is completed the embryo or eggs are loaded on a loading device and submerged immediately into liquid nitrogen. They are stored in liquid nitrogen at all times (-196)
The number of embryos depends on the stage of their development:
Once vitrified, we will know about their survival only when they have been thawed for the procedure. Though, if vitrified. properly they will remain in the same state or grade for years.
No even though you are 38 years old, your embryos are still of a younger woman as they were vitrified earlier. Your current age will have no impact.
No, there has been enough research and evidence to prove that vitrification is a safe technique.
They are similar to the fertilization rates in fresh oocytes.
If you are married, have a male partner but wish to postpone pregnancy, I would recommend you freeze your embryos.
Only mature oocytes after denudation are vitrified. Immature oocytes are discarded; that is probably why your clinic has frozen only 12 MII.
Each straw which contains embryos is labelled meticulously with your name and ID. Documentation is very important in an embryology lab.
No, once the straw is thawed, all embryos will have to be removed. We don’t have the option of removing one or two embryos from the straw. However, you may discuss the option of refreezing the embryos with your embryologist and clinician.